I doubt it. Reboot Ben doesn’t know much about his aliens’ species.
He does call them wheels rather than rollers tho. Even if he knew that he could take them off, it wouldn't be helpful since it slows him down, and he knows better than messing with his aliens
He gets that knowledge by the alien. Such as by Grey Matter, he gets the knowledge about the things which he does not know. The DNA of aliens present in the Omnitrix even has knowledge. In this the DNA of Kineceleran in the Omnitrix also has the knowledge because Azmuth had collected the DNA of a Kinceleran who has the knowledge about his powers: not a newly born Kinceleran.
^*In this way, the DNA of...
Well, an episode had him say “What do you mean? He’s (XRL8) got wheels.” so this is answered. Still don’t know if he knows that he can take them off though.