The Negative 10 appeared in the two-part episode, Ben 10 vs. the Negative 10. They were led by Driscoll, a former Plumber who was dismissed due to his evil actions and became the Forever King, leader of the Forever Knights.
- Driscoll (leader)
- Forever Ninja
- Dr. Animo
- Acid Breath
- The Negative 10 are similar to the Spider-Man villain team the Sinister Six. Like the Sinister Six being a group of Spider-Man villains desiring revenge against the wall-crawler, the Negative 10 is a villain team composed of villains Ben Tennyson has fought that has been brought together so they could have a chance to defeat him.